Malaysian Malay women face unique challenges in balancing sun exposure benefits and risks amidst religious attire requirements and societal ideals of fair skin. This study determines vitamin D status and its associations with sun exposure and dietary vitamin D intake among Malay female office workers in Kuala Lumpur, Malaysia. A total of 147 women (mean ± SD age: 36 ± 6.4 years) completed questionnaires on sun exposure and dietary vitamin D intake. Skin colour was assessed using a reflectance spectrophotometer, and serum 25-hydroxyvitamin D (25OHD) levels were analysed via enzyme-linked immunoassay. Results showed a mean ± SD 25OHD level of 35.2 ± 8.0 nmol/L, with only 9.6% (n=14) exhibiting sufficient levels (> 50 nmol/L). Most participants had skin types II (58.5%) and III (31.3%), with limited outdoor body surface area exposure (10% BSA). Sunscreen use was prevalent only during work (59.9%), while umbrellas were uncommon. Median dietary vitamin D intake was low (5.2 µg/day), with 94.6% failing below the recommended intake (> 15µg/day). Significant positive correlations were found between serum 25OHD levels and sun exposure index (rs = 0.30, p=0.001) and dietary vitamin D intake (rs = 0.27, p<0.001). Multiple linear regression analysis revealed that every unit increase in sun index corresponded to a 2.74 nmol/L rise in serum 25OHD, while a 1 µg increase in dietary vitamin D intake was associated with a 0.25 nmol/L increase. These findings highlight the high prevalence of insufficient vitamin D status within this population, emphasising an urgent need for targeted public health interventions.